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Image Search Results
Journal: The FASEB Journal
Article Title: Tmx2 Maintains Mitochondrial Function to Support Preimplantation Embryogenesis
doi: 10.1096/fj.202500640r
Figure Lengend Snippet: FIGURE 6 | Tmx2 knockdown inhibits mitophagy and autophagy in morula-stage embryos. (A-D) Immunostaining for PINK1 (A), PARKIN (B), MAP1LC3B (C), and LAMP1 (D) in control and Tmx2-knockdown embryos. Scale bars: 75 μm. (E-H) Quantification of immunofluorescence inten- sity for PINK1 (E), PARKIN (F), MAP1LC3B (G), and LAMP1 (H) in control and Tmx2-knockdown morula-stage embryos. Sample sizes: PINK1: Control = 16, siRNA2 = 14, siRNA3 = 19; PARKIN: Control = 21, siRNA2 = 20, siRNA3 = 23; MAP1LC3B: Control = 22, siRNA2 = 20, siRNA3 = 21; LAMP1: Control = 30, siRNA2 = 32, siRNA3 = 31. Statistical significance: **p < 0.01, ***p < 0.001 and ****p < 0.0001.
Article Snippet: The primary antibodies used included: rabbit anti- TMX2 (Origene, TA341391, 1:50), goat anti- OCT4 (Abcam, ab27985, 1:200), mouse antiCDX2 (Biogenex, MU392A- UC, 1:100), goat anti- SOX17 (R&D Systems, AF1924, 1:100), rabbit anti- NANOG (Abcam, ab80892, 1:50),
Techniques: Knockdown, Immunostaining, Control, Immunofluorescence